The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing
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چکیده
منابع مشابه
The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing
The etiologic agents for melioidosis and glanders, Burkholderia mallei and Burkholderia pseudomallei respectively, are genetically similar making identification and differentiation from other Burkholderia species and each other challenging. We used pyrosequencing to determine the presence or absence of an insertion sequence IS407A within the flagellin P (fliP) gene and to exploit the difference...
متن کاملBurkholderia pseudomallei and Burkholderia mallei: Melioidosis and Glanders
The genus Burkholderia is currently composed of many species, but only three are notable pathogens for humans or animals: the former cepacia complex (described in Chapter 220) pseudomallei (the agent of melioidosis), and mallei (the agent of equine glanders). All three are aerobic, nonsporulating, straight or slightly curved gram-negative bacilli that were formerly placed in the genus Pseudomonas.
متن کاملClear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
BACKGROUND A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. tha...
متن کاملDevelopment of Burkholderia mallei and pseudomallei vaccines
Burkholderia mallei and Burkholderia pseudomallei are Gram-negative bacteria that cause glanders and melioidosis, respectively. Inhalational infection with either organism can result in severe and rapidly fatal pneumonia. Inoculation by the oral and cutaneous routes can also produce infection. Chronic infection may develop after recovery from acute infection with both agents, and control of inf...
متن کاملMultiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei
BACKGROUND Burkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by travelers from unsuspected regions, also occur elsewhere. Rapid, sensitive methods for identification of B. mallei and B. pseudomallei are urgentl...
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ژورنال
عنوان ژورنال: International Scholarly Research Notices
سال: 2014
ISSN: 2356-7872
DOI: 10.1155/2014/109583